| Location | Milepost A | Notes |
|---|
| East | West |
|---|
| Ford | 0.3 | West End Double Track. Bristol Yard Limit. |
| Universal Siding | 3.4? | 3.5 | Universal Steel. |
| Vance | 6.0 | 6.1 | |
| ??? | 8.8 | 8.7? | |
| Bluff City | 11.8 | 11.9 | |
| Bluff City SAD | 12.4 | HBD-DED. Next to State Route 44. |
| Curtis | 13.7 | East End Siding. |
| Piney Flats | 15.7 | West End Siding. End CTC. TWC west to Sand Valley. |
| ??? | 18.6 | 18.5 | |
| Watauga | 20.8 | 20.? | |
| Johnson Ciy Chemical | 23.? | 23.3 | |
| Carnegie | 23.8? | 24.0? | Yard |
| West Siding | 26.? | 26.? | |
| Johnson City SAD | 27.2 | HBD-DED. |
| Johnson City Metals | 29.4 | 29.? | |
| Jonesborough | 31.6 | 31.? | |
| Sand Valley | 35.9 | East End Siding. TWC East to Piney Flats |
| Telford | 37.9 | West End Siding. TWC west to Jones. |
| Telford SAD | 40.3 | HBD-DED. |
| Washington College | 40.4 | 40.3 | |
| Limestone | 43.8 | 43.? | |
| Chuckey | 46.4 | 46.? | |
| East Afton | 49.? | 49.? | |
| West Afton | 50.? | 50.? | |
| Junk Yard | 52.4? | 52.9? | |
| Afton SAD | 52.9 | HBD-DED. |
| 54.2 | 54.? | |
| Greeneville | 56.? | 56.? | |
| ??? | 58.? | 58.? | |
| Jones | 62.3 | East End Siding. TWC east to Telford. Begin CTC. |
| Rader | 64.4 | West End Siding. TWC west to Justice. |
| Mosheim SAD | 66.8 | HBD-DED. |
| Midway | 66.8 | 66.9 | |
| Walmart | 68.? | 68.? | |
| Mohawk | 71.2 | 71.? | |
| Deer Valley | 73.2 | 73.? | |
| Bulls Gap | 75.6 | 75.7 | East End Siding (spring switch), East leg of Wye. |
| Justice | 76.0 | Crossover. West leg of Wye. TWC east to Rader. Connection to Appalachia District. |
| Kite | 77.6 | West End Siding. |
| Whitesburg SAD | 79.0 | HBD-DED. |
| Whitesburg | 80.8 | 80.7 | |
| Russelville | 83.0 | 83.1 | |
| Berkline | 86.? | 86.3 | aka "Motorcycle Shop" |
| Morristown SAD | 87.1 | HBD-DED. |
| Pickens | 89.0 | East End Double Track. |
| New Line | 91.4 | Crossover (2->1 EB). Junction w/New Line to Leadvale and Asheville. |
| Coulter | 92.7 | |
| Alpha | 94.1 | East End Double Track. |
| Talbot | 97.2 | 97.1 | |
| Talbot SAD | 98.9 | HBD-DED-HWD. |
| Keister | 100.0 | East End Double Track. |
| Jefferson City | 102.? | 102.3 | |
| New Market | 104.4 | 104.? | |
| Friends | 107.1 | Crossovers. |
| Hodges | 110.2 | West End Double Track. |
| Hodges SAD | 111.1 | HBD-DED. |
| Old Hodges | 112.? | 112.3 | |
| Strawberry Plains | 114.? | 114.7 | |
| Mascot | 116.? | 116.9 | |
| Mascot DED | 111.1 | DED only, voice alarm. |
| Roseberry | 119.2 | East End Double Track. |
| East End Sevier Yard | 121.6 | East End of Sevier Yard. Crossover. |
| Lizzie | 122.2 | Yard Limit. |
| West Sevier | 125.0 | West End of Sevier Yard. |