| Location | Milepost NB | Notes |
|---|
| West | East |
|---|
| Walton | 297.6 | Junction with Christiansburg District.
Controlled by New River Distpatcher |
| Tyler | 297.9 | Controlled by New River Dispatcher |
| Plum Creek | 298.9 | East end of Radford Yard |
| Radford | 300.7 | Crossover |
| JC | 302.1 | West end of Radford Yard.
Junction with Radford Branch |
| New River TSA | 302.6 | HBD-DED, High-Wide |
| name? | 304.7 | 304.6 | Visible along US 11 between Dublin and Fairlawn. |
| name? | 306.9 | 306.8 | Visible along US 11 between Dublin and Fairlawn. |
| Wysor | 309.2 | East end of siding. Just west of VA Rt. 100 overpass |
| Newbern | 310.5 | West end of siding, east of Volvo truck plant |
| name? | 313.1 | 313.2 | |
| name? | 315.1 | 315.2 | |
| name? | 317.1 | 317.2 | |
| Pulaski TSA | 317.2 | HBD-DED |
| name? | 318.9 | 318.8 | |
| Clark | 322.1 | East end of siding |
| Gunton Park | 323.3 | West end of siding |
| name? | 326.7 | 326.8 | |
| Max Meadows TSA | 329.7 | HBD-DED |
| name? | 329.9 | 329.8 | |
| Kent | 333.1 | 333.2 | |
| Stones Mill | 336.3 | East end of siding |
| Wytheville | 337.4 | West end of siding |
| name? | 340.3 | 340.4 | |
| Grubb TSA | 340.6 | HBD-DED |
| name? | 343.3 | 343.4 | |
| Crockett | 345.8 | East end of siding. |
| Duncan | 347.8 | West end of siding. Named after a former dispatcher. |
| Rural Retreat | 349.6 | Next to station. |
| name? | 351.9 | 352.0 | |
| Groseclose TSA | 353.5 | HBD-DED |
| Groseclose | 354.7 | 354.8 | |
| Atkins | 357.7 | 357.8 | Just east of Route 626 (East of Atkins Tank Road) |
| name? | 359.9 | 359.8 | West of Route 622 overpass and Martlett Mouldings |
| Schuleen | 362.9 | East end of siding |
| Marion | 364.1 | West end of siding |
| Marion TSA | 365.4 | HBD-DED |
| Copenhaver's Crossing | 366.5 | 366.4 | |
| Jessie's Cut | 369.5 | 369.6 | |
| Seven Mile Ford | 372.1 | 372.0 | |
| Chilhowie | 375.1 | 375.0 | |
| Snapp | 377.9 | 377.8 | |
| Chilhowie TSA | 378.0 | HBD-DED |
| Glade Spring | 380.4 | East end of siding |
| Washington | 381.7 | West end of siding |
| Emory | 384.3 | 384.2 | |
| name? | 387.5 | 387.6 | |
| Abingdon TSA | 390.4 | HBD-DED |
| Hayter | 390.7 | 390.6 | |
| Litchfield | 393.2 | East end of siding |
| Abingdon | 393.9 | West end of siding, at Preston St. |
| name? | 396.9 | 396.8 | Along route 645. |
| Wyndale | 399.7 | Just west of Astor Road, off Route 645/Wyndale Road. |
| Wallace TSA | 402.6 | HBD-DED |
| Wallace | 402.7 | 402.6 | Along Route 645 |
| Cecil | 404.9 | 404.8 | |
| Bristol | 407.3 | East End of Bristol Yard. Connection to Central Division at west end of yard. |