| Frequency | District(s)/Terminal(s) |
| 160.440 | Roanoke District (Roanoke to Shenandoah, VA) |
| Princeton-Deepwater District, Guyandotte River Branch |
| Winston-Salem District (Roanoke to Winston-Salem, NC) |
| 160.650 | Pocahontas District (Bluefield to Williamson) |
| Kenova District (Williamson to Portsmouth) |
| 161.190 | Christiansburg District |
| Whitethorne District |
| Lynchburg District (Ex-N&W from Roanoke to Crewe) |
| AltaVista District (Ex-VGN from Roanoke to Crewe) |
| Auville Yard, Williamson Yard, Elmore Yard |
| 161.250 | Pulaski District (Radford to Bristol) |
| Roanoke Yard, Bluefield Yard, Bristol Yard |
| 161.490 | Clinch Valley District (Bluefield to Norton) |
| Dry Fork Branch (Cedar Bluff to Iaeger) |
| 160.950 | Danville District (Lynchburg to Linwood) |
| Washington District (Alexandria to Lynchburg) |
| Appalachia District (Bulls Gap, TN to Appalachia, VA) |
| Knoxville District (Bristol to Knoxville) |
| 160.245 | Knoxville/Appalachia Districts (Dispatcher to Train) |
| 160.830 | Knoxville/Appalachia Districts (Train to Dispatcher) |